Our it staff was able to power on the smart cycler and included. Rox solution is provided separately for use with machines that. For the applied biosystems 7500 real time pcr system and the stratagene mx3000p, the use of rox reference dye lmp is recommended. Smartcycler system is a leading realtime pcr testing platform for research hospitals, university labs and government agencies. The cepheid smartcycler ii is a modular real time pcr system. Alle biorad cycler, cepheid smart cycler, eppendorf mastercycler ep realplex, illumina. Honestly, we have been working during the lasts years basically with the roche system, with good result. It is not required for use with smart cycler or lightcycler realtime instruments. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by. Cepheid smart cycler 9000330 automated realtime pcr. The lightcycler 96 system embodies roches expertise over the last decade in developing and providing highperformance qpcr systems that enable research breakthroughs. The smartcycler system performs integrated amplification and detection automatically in a single step, with. Applied biosystems simpliamp thermal cycler thermo.
Parameters involved in the conversion of realtime pcr assays. The optical system of the unit is centered around the icore, a solid state module which does not require. The applied biosystems simpliamp thermal cycler is an easyto use, compact, and accurate thermal cycler. After successfully calibrating your realtime pcr instrument, the reaction plate containing your calibration. Real time pcr is performed in a smart cycler cepheid, sunnyvale, ca using the lightcycler dna master sybr green i dye intercalation assay roche, indianapolis, in, although there are many other manufacturers for equipment and reagents. Thermal cyclers cepheids smart cycler thermal cycler system monitors the accumulation of pcr products by performing both amplification and. Jan 12, 2017 cepheid smartcycler ii thermal cycler automated realtime pcr system 10268. Each of these randomaccess, realtime, modular pcr processing units can test 16 different protocols simultaneouslythermal cyclers are used to amplify segments of dna via the. A batterypowered notebook thermal cycler for rapid multiplex realtime pcr analysis phillip belgrader, steve young, bob yuan, michael primeau, lee a. Honestly, we have been working during the lasts years basically with the roche system, with. Realtime pcr detection of shigatoxin producing escherichia coli stec, serotype o157 and non o157 in fresh produce and food with nono157 isolation and identification revision. Quick reference for real time rt pcr influenza a assays for subtype h5 or h7 detection for ab7500 fast and cepheid smart cycler. The cartridgebased assay detects dna of chlamydia trachomatis and neisseria gonorrhoeae. Eu 2x q pcr master mix is a is a readytouse reagent with all the essential components for quantitative real time pcr qpcr except primers and templates.
Smartchip realtime pcr cycler smartchip qpcr software user manual 071619 takara bio usa, inc. In order to successfully use duallabeled probes containing cal fluor andor quasar dyes in realtime, quantitative pcr qpcr multiplexed assays, certain realtime pcr instruments must first be calibrated to recognize the pure dye spectra. Evaluation of the smartcycler ii system for realtime. The following outlines a rapid and robust method for detecting. We designed, optimized, and extensively tested several sensitive and speci. The ability of the portable cepheid smartcycler real time pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the analyses of nasal swab and serum samples from experimentally infected animals with those obtained from the real time pcr assay currently in use in the laboratory.
The optical system of the unit is centered around the icore, a solid state module which does not require warm up, maintenance, or. Cal fluor and uasar yes thermal cycler spectral calibration. Qiagens new realtime pcr cycler, the rotorgene q, provides outstanding. Discover the new lightcycler 96 realtime pcr system a compact and smart device thats the perfect companion for qpcr newbies and experts. Smart cycler ii can mimic the conditions of other realtime cyclers. The analytical sensitivity of our rt pcr assay was determined to be pcr was 100%. Csu surplus property is selling a cepheid smart cycler ii td real time pcr system previously used in a university setting. The master mix includes maxima hot start taq dna polymerase and dntps in an optimized pcr buffer. Methods genomic dna was extracted from whole blood.
Detection of food pathogens using the smart cycler ii gene. The cepheid smartcycler system is an integrated dnarna amplification and. The assay is a rapid realtime pcr nucleic acid amplified test. Methods such as realtime pcr rtpcr provide rapid and reliable detection that can supplement attempts to culture p. Delivers highly accurate and consistent test results in as little as 20 minutes. Evaluation of the portable cepheid smartcycler realtime. The smart cycler ii system is a rapid, realtime thermal cycler used for identifying dnarna from prepared biological samples. Evaluation of the portable cepheid smartcycler real time pcr machine for the rapid diagnosis of footandmouth disease hearps, a, zhang, z and alexandersen, soren 2002, evaluation of the portable cepheid smartcycler real time pcr machine for the rapid diagnosis of footandmouth disease, veterinary record, vol. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smartcycler system software program the software on the computer provided with the smart. A singletube realtime fluorogenic reverse transcription rtpcr with the smartcycler instrument smartcycler rtpcr for influenza a virus detection was evaluated. This is a traditional 510k submission requesting market. We offer a thermal cycler for every challenge, application, and budget.
It is not required for use with smart cycler or lightcycler real time instruments. Evaluation of cepheid smartcycler and euroclone duplicareal. Thermal cycler dice real time is a compact system for 96 plate type with highperformance hardware and easytouse software. Sensitive chargecoupled device ccdbased optics enable accurate quantitation over a dynamic range of 9 orders of magnitude. Direct immunofluorescence antibody staining dfa and primary rhesus monkey kidney cell culture were performed onsite at yalenew haven hospital. Comparison of smartcycler realtime reverse transcriptionpcr. Real time pcr system, the use of rox reference dye lsr 50x is recommended. Cepheid smart cycler 9000330 automated realtime pcr system. Method 1609 describes a quantitative polymerase chain reaction qpcr procedure for the detection of dna from enterococci bacteria in ambient water matrices based on the amplification and detection of a specific region of the large subunit ribosomal ribonucleic acid rna gene lsrrna, 23s rrna from these organisms. The smart cycler ii system is a rapid, real time thermal cycler used for identifying dnarna from prepared biological samples.
Cepheid smartcycler system software license agreement license grant. Csu surplus property is selling a cepheid smart cycler ii td realtime pcr system previously used in a university setting. Manual for the design and use of oligonucleotide specialty fluorescent probes. United states department of agriculture agricultural. Evaluation of the portable cepheid smartcycler realtime pcr. Pcr thermal cyclers from applied biosystems thermal. The objectives of the study were to compare the smartcycler ii system results with those of standard cell culture, to compare the smartcycler ii system results with those of a dedicated pcr facility, and to establish the smartcycler ii system as a pcr method for detecting viral and chlamydial dna from ocular specimens. Comparison of smartcycler realtime reverse transcription. Thermal cycler spectral calibration instructions why is spectral calibration necessary. Our it staff was able to power on the smart cycler and included laptop.
This study evaluated the conversion of a real time pcr assay from the abi prism 7700 instrument abi, foster city, ca to the cepheid smartcycler ii cepheid, sunnyvale, ca using taqman detection chemistry. The presence of amplified product is confirmed when the fluorescent signal exceeds a userdefined threshold. Pdf evaluation of the portable cepheid smartcycler realtime. Rida cycler the multiplex realtime pcr thermocycler duration. Smartcycler instrument in a protocol comprising rt and pcr. This block upgrades any existing smartcycler to accommodate an additional 16 reaction sites for real time pcr analysis. Cepheid asrs are also delivered with an internal control to ensure optimum reliability by confirming that negatives are truly negative not inhibited.
Cepheid smart cycler ii rt pcr model sc2 with 16 well block, including computer with software. Cepheid smartcycler ii thermal cycler automated realtime pcr system 10268. Chlamydia trachomatis, smartcycler, cepheid, euroclone, evaluation, realtime pcr. Cepheids smartcycler instrument is the only random access, modular real time. The assays are based on taqman 3minor groove binder chemistry and were performed on both the rapidcycling roche lightcycler and the cepheid smart cycler platforms. Kits chemistries adapted to slow ramp rates can be used in the smart cycler ii martina fricker qpcr 2005 07. Optimizing and analyzing realtime assays on the smartcycler ii. We recommend this system as a perfect realtime pcr instrument with confidence. Available realtime pcr cyclers gene quantification. In our study, realtime rtpcr was performed in one tube with the smartcycler instrument. Cepheid smartcycler 16 site automated realtime pcr system. Used cepheid smartcycler ii thermal cycler automated real. Download user guide rest 2009 software user guide english pdf. Pcr instrument on the market with up to 96 sites that can each run a different.
Details about cepheid smart cycler 9000330 automated realtime pcr system. Detection of food pathogens using the smart cycler ii. Smartcycler dx system processing block, diagnostics software, user manual. The smartcycler system performs integrated amplification and detection automatically in a single step, with results in real time as pcr reactions are run. Computer is windows 2000 with cepheid smart cycler. Cepheid smartcycler ii thermal cycler automated realtime pcr. The following outlines a rapid and robust method for detecting the human multidrug resistance gene mdr1 snp c3435t using real time polymerase chain reaction pcr. Methods such as real time pcr rt pcr provide rapid and reliable detection that can supplement attempts to culture p. Pdf the ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and. The analytical sensitivity of our rtpcr assay was determined to be pdf, sign in to an existing. The assay was performed with the cepheid sunnyvale, calif. The cepheid genexpert xpert ctng assay was us fdacleared in december 2012. Method 1609 describes a quantitative polymerase chain reaction qpcr procedure for the detection of dna from enterococci bacteria in ambient water matrices based on the. Cepheid smart cycler ii td realtime pcr system govdeals.
Amplification and detection on the cepheid smart cycler. Find out why cepheid s solutions are a top choice for fast, accurate, and easy diagnostic testing. Cepheid smartcycler ii thermal cycler automated realtime. Evaluation of the portable cepheid smartcycler realtime pcr machine for the rapid diagnosis of footandmouth disease article pdf available in the veterinary record 15020. By automating much of the testing process, and making each reaction site individually programmable, it is the fastest, easiest to use, and most flexible system now on the market. The laptop connects to the cycler and recognizes it. Ridacycler the multiplex realtime pcr thermocycler duration. Each asr product is designed for rapid results on realtime pcr instruments like the cepheid smartcycler system. Takara bio launched a realtime pcr instrument for 96 plate. Available realtime rtpcr cycler and kinetic pcr machines. The use of rox reference dye lsr or lmp is optional. Cepheids smartcycler instrument is the only random access, modular realtime. The ability of the portable cepheid smartcycler real time pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the analyses.
Realtime pcr is performed in a smart cycler cepheid, sunnyvale, ca using the lightcycler dna master sybr green i dye intercalation assay roche, indianapolis, in, although there are many. The sc2000n31 is an addon block for the cepheid smartcycler ii real time thermal cycler. Detection of biological threat agents by realtime pcr. Morrison 42015 page 12 rapid, realtime thermal cycler used for identifying dnarna from prepared biological samples. A batterypowered notebook thermal cycler for rapid. It is fdacleared for use in female endocervical swabs, patientcollected vaginal swabs and for female and. Pcr thermal cyclers from applied biosystems thermo. Elegant design, smart choice now cloudenabled for secure remote access. Quick reference for realtime rtpcr influenza a detection using the iav m 2009 assay for ab7500 fast and cepheid smartcycler ii wiav0045 4. Quick reference for real time rt pcr influenza a detection using the iav m 2009 assay for ab7500 fast and cepheid smartcycler ii wiav0045 4. In our study, real time rt pcr was performed in one tube with the smartcycler instrument. Please refer to the smartcycler operator manual, appendix b, and the application note user. The abi prism 7700 instrument using taqman chemistry was one of the first real time pcr instruments to become commercially available. Thermo scientific maxima probe qpcr master mix 2x is an universal readytouse solution for quantitative realtime pcr and twostep realtime rtpcr on most realtime pcr machines.
Post a free request for proposal for a cepheid smartcycler ii thermal cycler automated realtime pcr system and get quotes from our gold service dealers. A singletube real time fluorogenic reverse transcription rt pcr with the smartcycler instrument smartcycler rt pcr for influenza a virus detection was evaluated with 238 respiratory specimens. We adapted and optimized a previously described mitochondrial genebased rt pcr assay for use with a cepheid smartcycler v. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the. Cepheid smart cycler ii rtpcr model sc2 with 16 well block, including computer with software. Realtime pcr assays for detecting biological warfare agents have been developed on the r. Here we discuss tips and guidelines to optimize smartcycler pcr protocols. During pcr cycling, optical signals from two sequence specific fluorescent probes within the reaction are monitored in real time.
As many as six of these blocks can be added to a single smartcycler unit, providing up to 96 independently controllable sites for read more. The optical system of the unit is centered around the icore, a solid state module which does not require warm up, maintenance, or normalization dye. Method 1611 describes a quantitative polymerase chain reaction qpcr procedure for the detection of dna from enterococci bacteria in ambient water matrices based on the amplification and detection of a specific region of the large subunit ribosomal rna gene lsrrna, 23s rrna from these organisms. It contains eutaq polymerase in an optimized buffer with dsdna specific sybr green fluorescent dye. Cepheid smartcycler ii thermal cycler realtime pcr system. Background fluorescence is a property of real time pcr and can be defined as. The cepheid smart cycler dx system is an integrated nucleic acid amplification and detection instrument system based on the proprietary microprocessorcontrolled icore module.
Up to 16 independently programmable reaction sites, can simultaneously run. Computer is windows 2000 with cepheid smart cycler version 2. Review of use of a new rapid realtime pcr, the cepheid. For real time applications, cepheids real time pcr reaction tubes designed specifically for use with the cepheid smart cycler system, ensure rapid thermal transfer and excellent optical. Smallpox and panorthopox virus detection by realtime 3. Cepheid s genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. Rapid diagnostics for chlamydia trachomatis and neisseria gonorrhoeae are desirable so that patients can be treated while they are still in the clinic or doctors office. Smart modular designs the myiq and iq5 realtime pcr detection systems are 96well modular upgrades to the icycler thermal cycler, which delivers excellent thermal performance and thermal gradient capability. For real time applications, cepheids real time pcr reaction tubes designed specifically for use with the cepheid smart cycler system, ensure rapid thermal transfer and excellent optical sensitivity. For the applied biosystems 7500 real time pcr system and the stratagene mx3000p, the use of rox.
The smartcycler dx system is a rapid, realtime thermal cycler used for. Real time pcr offers several advantages over traditional pcr methods. Problems with fast ramp rate chemistry not compatible. The reagents for the amplification reaction on the cepheid smartcycler were provided in the euroclone duplica real time dual easy ct assay. The heart of the smart cycler system from cepheid of sunnyvale, calif.
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